"For our research, EthoVision XT is an essential tool for sophisticated analysis of behavior as it relates to neural circuit function"
Garret Stuber, University of North Carolina at Chapel Hill, UNC Neuroscience center, Chapel Hill, USA
“EthoVision software has dramatically improved all areas of behavior testing”
Joshua Matulonis, Research Foundation at Stony Brook University, USA
“Tracking software is in the forefront of scientific studies, but various software platforms are either not reliable or very difficult to use. EthoVision XT provides extremely reliable software with an interface that allows everyone to easily track animals” - Erik R. Duboue, New York University, USA
“The Heartbeat detector is magic !”
Prescilla Perrichon, IFREMER – La Rochelle – France
"A great tool for tracking very small insect larvae under IR conditions"
Nick Birch, Scottish Crop Researcher Institute, Dundee, UK
"We successfully use EthoVision and plan to use The Observer in practice for our students"
Marina Pleskacheva, Moscow State University, Lab. Of Physiology, Genetics and Behavior, Moscow, Russian Federation
"EthoVision saves time, perhaps lives. In effect, PhD students can do more research in less labor-intensive time"
Samuel Adeosun, University of Mississippi Medical Center, Jackson, USA
"EthoVision XT is a great product, EthoVision just gets better and better"
Thomas van Groen, University of Alabama at Birmingham, Cell Biology department, Brimingham, USA
“EthoVision is user-friendly and incredibly versatile: it can be used for a wide variety of behavioral experiments, across different lighting conditions (and coat colors), and can track from a range of distances between camera and subject.” - Jerome Pagani, Behavioral Neuroscientist, USA
"EthoVision allows me to track videos accurately and reliably. Now I can use the hours that I used to spend with timers, pen and paper working on other areas of my research"
Jacqueline Womersley, University of CapeTown, Department of Human Biology, Cape Town, South Africa
“This is the best tracking system for open field behavior!”
David Eilam, Tel-Aviv University, Zoology Department, Israel
“A truly useful software to understand animal behavior”
Allan V. Kalueff, Department of Pharmacology, Tulane University Medical School, USA
“EthoVision XT has a convenient tracking/video system with excellent data analysis options and it is fairly intuitive to use”
Diane Lim, University of Pennsylvania, USA
“Really nice tracking program”
Matt Andrzejewski, University of Wisconsin-Madison, USA
“EthoVision XT has allowed our lab to collect rich behavioral data that would have been impossible with our analog system”
Joshua Herrington, Florida International University, USA
“This set of programs allows for flexibility of behavioral observations, scoring and data analyses”
Craig Kinsley, University of Richmond, USA
“EthoVision XT is a powerful tool to analyze insect and spider behavior including tiny little dwarf spiders. Despite of the numerous settings, the intuitive program design allows students to quickly get into the program.”
Roman Bucher, University of Koblenz-Landau, Germany
“DanioVision is an excellent system! We have been using it for two years now, to study both medaka and trout early life stages, and got great results.”
Prof. Cachot, University of Bordeaux, France

What’s new in EthoVision XT 10

EthoVision XT 10: coming soon! A peek at the new features:


automatic detection of rat behaviorAutomatic Rat Behavior Recognition

Revolutionize the way you work with automatic recognition of rat behavior. This new system will make your research more efficient and objective. It is as accurate as a human observer and has a better repeatability, giving you consistent results every time. No extra training is needed when using a different experimental set-up or strain of rats. The system is also tireless, allowing you to perform ‘round the clock’ observations in an efficient manner.

Recognized behaviors include:

  • feeding
  • grooming
  • jumpingautomatic recognition of rat behavior
  • rearing against the wall
  • unsupported rearing
  • twitching
  • sniffing
  • walking


Batch acquisition and analysis

Save valuable time by letting EthoVision XT track all your video files in one go and run automatic analysis on all your data with one push of the button!


Group statistics and charts

View the results of groups in tables and in graphs to get an immediate feel for your data. Compare treatment groups alongside each other or look at the differences within treatment groups over time.



This unique built-in feature gives you an intuitive presentation of your data, and enables you, in one glance, to answer the question ‘In which part of the arena did the animal spend most of its time?’ You can select data and compare heatmaps of groups or individuals. Of course, you can export them for use in scientific publications and presentations.


Series of trials from live video feed

Acquire a series of trials automatically. Take a water maze test, for example. Set each trial to last for 60 seconds, or until the animal has reached the platform, and set an interval of 10 minutes in between trials to replace the animal.


EthoVision XT runs on Windows 7 and Windows 8, on 32 and 64 bit computers.


Other recent new features (EthoVision XT 9)


EthoVision XT activity detection  freezing behavior Activity detection: sitting still versus freezing

An animal can be sitting still, but that does not automatically mean that it is freezing. To solve this, EthoVision XT has a new method for activity detection. Now you can now detect body displacement and orientation of the animal, as well as activity levels within the arena. This combination is really powerful for detailed analysis of fear conditioning or operant tests. 

The new activity detection is also very suitable to detect zebrafish embryonic tail flicks or convulsion behavior, or for the heartbeat monitoring of zebrafish larvae. The heartbeath detection (free software with EthoVIsion XT 9) extracts a power spectrum from your EthoVision XT data to identify the heartbeat. 

Another great advantage of activity detection is that it even  works  in situations where detection is dificult due to poor lighting or light animals on a light background (or dark animals on a dark background).

EthoVision XT Activity Detection in action!


Danio rerio Heartbeat detection in zebrafish larvae

Studies on new drugs are often discontinued due to negative side effects of those drugs on the heart. Cardiovascular defects and cardiotoxic effects of compounds can be easily measured in zebrafish larvae thanks to their transparency, small size, and ease of manipulation. With the EthoVision XT Heartbeat Detector this now becomes very easy and can be done without any fluorescent labeling technologies. 

How? EthoVision XT comes with Activity Detection, a new method to measure the changes in pixels within an area between samples. When this area is the heart region of a zebrafish larva, the heart activity is detected. 

Heartbeat detector

This data is used by the Heartbeat Detector to calculate the  dominant frequency and determine the heart rate of the zebrafish larva. 

All you have to do is record the activity of the zebrafish larval heart area and the heart rate is calculated for you!


Your experiment, your choice in equipment

In addition to proven solutions with analog cameras, EthoVision XT supports a range of digital cameras, such as FireWire or GigE. These are particularly interesting in situations that require a high frame rate (quickly moving animals such as fish) or high resolution (small animals such as larvae in a large arena). Low-end digital cameras are useful if you want to create a cost-efficient solution for less demanding tests. 

Picking your set-up usually involves more than just choosing a camera. Some experiments (operant tasks or conditioning) require interactions with hardware. That is why EthoVision XT has the Trial & Hardware Control Module; to control external equipment such as pellet feeders, lights, sound generators, and more, from within the software. By using EthoVision XT, you can even use behavioral based events in your operant task. This is taking operant conditioning to the next level!

3 examples of EthoVision XT video tracking

Batch acquisition: analysis overnight

EthoVision XT allows you to acquire the tracking data of your animal live, but in some cases it is more practical to create video files of your experiments, and process these later. For example, when EthoVision XT is being used for other experiments. Processing these video files has become much easier, because you can now run a batch acquisition. Simply connect the video files to the according trials in your trial list. Now you can acquire your data overnight!