A simple method for measuring heart-rate in Guinea pigs during a standard handling procedure

R.D. Beer

Department for Animal Physiology, University of Bayreuth, Bayreuth, Germany

 

Introduction

In the course of a longitudinal study on social stress in female Guinea pigs [1] we developed a simple and noninvasive method to estimate the heart rate during a standard laboratory handling procedure. The method had to meet the following requirements:

Method

The investigated animals were familiar with a daily standard procedure which included weighing in a plastic basin since their first day of life. In order to avoid disturbing the animals we used this procedure as base for our heart rate measurement: The head of an electronic stethoscope (Bosch, Germany) was built in the weighing basin in a way, that it was directly under the chest of the Guinea pig (figs. 1 and 2). By using an underneath accessible adjusting device the stethoscopeís head could always be adjusted in an optimal position without touching the respective animal with the hands. The electronically amplified heart rate sounds and the animalís identification were recorded with a commercial two-speed dictation machine (Sanyo, Japan).

Figure 1. During the daily weighing procedure the Guinea pig remains calm in the basin.

Figure 2. Placing the animal in the heart-rate measuring basin. The device is placed under the animalís chest.

A measuring period of 30s proved to be sufficient for reliable results. The evaluation of the heart rate (beats per minute) was done with the help of the program The Observer (Noldus, The Netherlands). The heart beat sounds were played with half speed while a trained person had to press a key synchronously with the beats until the program ended the recording after 60 s.

Results

References

  1. Beer, R. (1997). Social stress in female guinea pigs exposed to lifelong daily change of social environment. In: Proc. Int. Congress of Stress: Stress of Life - Stress and Adaptation from Molecules to Man (Budapest, Hungary, 1-5 July 1997).
  2. Wagner, J.E.; Manning, P.J. (1976). The Biology of the Guinea Pig. Academic Press, New York.

Poster presented at Measuring Behavior '98, 2nd International Conference on Methods and Techniques in Behavioral Research, 18-21 August 1998, Groningen, The Netherlands

© 1998 Noldus Information Technology b.v.