Testing in a


The T-maze task is an investigation of spatial learning and memory. Subjects are often taught to discriminate between the two arms based on visual, olfactory, tactile or even auditory cues during consecutive trials. Subsequently, reversal learning or retention can be investigated. Alternatively the T-maze is sometimes used for place preference testing. The T-maze is similar to the Y-maze.

Testing in a T-maze

High quality mazes

The Noldus T-maze is specifically designed for working with rats or mice. Our standard design is made from grey non-reflective material, and is ideal for video tracking with EthoVision XT and is easy to clean. It also comes with three partitions so you can easily section off each arm.

We also offer a specific T-maze (cross maze) for zebrafish studies.

t maze illustrated

Made for you

The maze can be easily adjusted or built to your specifications. For example, you can choose a different color, a longer start arm, or add an IR backlight. All mazes are available in a cost-efficient package deal including a computer and a full EthoVision XT software license for video tracking. This license can also be used for the video tracking and automation of other behavioral tests.


Free e-book

Basic behavioral neuroscience in rodents

Want to test working memory in rodents? Or short term (spatial) memory in general. In our free basic behavioral neuroscience e-book we explain how these types of memory can be tested in a T-maze. Why and how do we effectively use the T-maze in rodents?


heatmap generator t maze ethovision

Video tracking

Performing your T-maze test with EthoVision XT video tracking allows you to easily get parameters like latency to enter the correct arm, number of transitions into each arm, time spent in each arm, and more. Using all three body points (nose, center, and tail base), you can acquire very accurate data.


Automation options

EthoVision XT has the ability to control hardware. For example, you can use automated doors that will open and close at set time points or based on the animal's behavior.


Alternative suppliers

Noldus mazes can be standard or custom build to your specifications, and are optimally suited for video tracking with EthoVision XT, and available in a package deal. Alternatively, we also offer mazes from several suppliers such as Ugo Basile and Maze Engineers (availability may depend on your location).


Relevant blogs

Zebrafish research: behavioral differences between wild-type strains

Zebrafish research: behavioral differences between wild-type strains

Often in animal research, animals with a certain genetic alteration are compared to a “wild-type”. One might assume that there is no differences between wild-types, but many different strains of wild-type animals are used.
5 proven ways to measure spatial learning in rodents

5 proven ways to measure spatial learning in rodents

What is spatial learning, why is it important and how do we measure it? Here we dive into 5 different behavioral tests that specifically measure spatial learning and memory in rodents.
Testing PCBs toxicity - behavior in zebrafish and their offspring

Testing PCBs toxicity - behavior in zebrafish and their offspring

PCBs are synthetic molecules that were used in transformers, electric motors, and more applications. It was quickly discovered that these molecules are toxic, and subsequently, they were banned.
Ready to kick start your research?
Want better insights and faster results? Contact us now to learn how we can help you!

Email address *

Organization *

Country *

Privacy policy *

Information and updates

I would like to receive high-quality content produced by Noldus that is relevant for me, such as blogs, product updates, customer stories, and events.