DanioVision is a complete system, including a fully functional EthoVision XT license and the Observation Chamber with white light, IR-light, and IR-sensitive camera. With this system you can perform a wide range of experiments. However, we also offer a range of add-ons to further broaden your experimental options.
Add-ons for DanioVision
A constant temperature is important in zebrafish research, especially during relatively long trials, having a large impact on mobility and other parameters.
For this reason we developed The Temperature Control Unit. Unlike competing systems where the temperature is measured in the control unit, our system controls the exact temperature of the water directly in the Observation Chamber. This way, you are sure that the water in the well plates is always at the desired temperature.
The DanioVision Temperature Control Unit is a flow-through system: water flows evenly underneath the well plate at the temperature of your choice. The temperature is the same all throughout the well plate.
In this video, you can see how the flow-through system works. (Blue dye is added to visualize the effect.) After you have set the temperature, the water will quickly spread out evenly underneath the well plate.
Zebrafish larvae display a robust startle response. Because this is mediated by neural pathways in a way similar to the process in higher vertebrates, startle response testing can be applied to a wide range of neuro-scientific studies.
The startle response can be evoked in different ways, such as via a light stimulus (standard in DanioVision) or a mechanical stimulus.
Tapping Device (a solenoid) is inserted at the bottom of the basin where your well plate or petri dish is placed in the DanioVision Observation Chamber. You can set the stimulus intensity on eight different levels. In the photo at the right, the cover plate is removed so you can see the built-in stimulus.
Like the White Light stimulus, the mechanical stimulus can be programmed in the EthoVision XT software (intensity, onset) and can be used in an automated analysis.
Optogenetics allow scientists to control the activity of specific neurons and study their downstream influence on a variety of biological processes, including behavior. In rodent studies this technique requires optical fiber implantation. Since zebrafish larvae are transparent, fiber optics are not needed. Instead, the correct wavelength (color) of light is required. Light simply needs to shine in the right direction, making it easy to study the role of specific neurons in behavior.
The Optogenetics Add-on provides a way to accurately control and precisely time the application of optogenetic stimulation. It is based on the Prizmatix Modular LED system and can consist of three different LED wavelengths that can work independently from each other.
Program with EthoVision XT
The Optogenetics add-on can be programmed and controlled using the EthoVision XT software included with your DanioVision system. You can configure onset and offset of the optogenetic stimulus based on time or the behavior of your larvae. Unlike in competing systems where only a single LED can be built in, and is controlled manually, this offers far better temporal precision, and adds efficiency to longitudinal studies. Like the White Light and mechanical stimulus, onset and offset of the stimuli are logged and can be used in your analysis.
You can also combine optogenetics with other DanioVision add-ons.
DanioVision Toplight Unit
Some studies require lighting from a more ‘natural position’ of light. For example, some animals experience conflicts in their innate orientation systems, when exposed to light from beneath. Lighting from above is also essential in some photobiology studies*. For these purposes, the Toplight Unit was designed.
Recent studies have shown that zebrafish are able to discriminate between different colors, and that wavelength and color influence their development, growth, and survival. These findings provide opportunities to test learning, memory, anxiety, and more.
Read more about color discrimination in zebrafish studies here.
The DanioVision Toplight Unit has a multifunctional design: one side consists of white LEDs, while the other side contains red, green, and blue LEDs. You can set the intensity of the white light to a maximum of 30,000 lux.
Besides being able to continuously run the Toplight unit, it can also be easily programmed and controlled based on timing or animal behavior using EthoVision XT.
You can combine the DanioVision Toplight Unit with other add-ons, such as the Tapping Device.
*Information kindly provided by Kristin Tessmar-Raible from the Max. F. Perutz Laboratories/ University of Vienna, Research Platform Rhythms of Life, Vienna Biocenter, Austria.
As research evolves, so do research needs. If you are interested in a custom solution, don’t hesitate to contact us!
One recent example of a custom solution is a light-dark grid, commonly used to investigate anxiety behavior in zebrafish. The grid is placed underneath the well plate (available for 6, 12, 24, 48 or 96 wells) and creates a dark and light half in each well. The grid is IR light translucent, leaving the tracking of the zebrafish unaffected.