• Clock genes

Dr. Zohar Ben-Moshe from the Yoav Gothilf Lab identified 14 light-entrained molecular clock genes in the zebrafish system using messenger RNA sequencing and microarray analysis. In this study (Nucleic Acids Research, 2014), genes dec1 and per2 were tested further and found to be essential for the light entrainment of the rhythmic locomotor activity of larvae. 

Video tracking experiment

The activity of larvae (dec1 knockdown, per2 knockdown, and control groups) was monitored in a DanioVision system. To entrain rhythms of locomotor activity, 5dpf larvae were exposed to 3 hours of light, after which they were monitored under dim light conditions.

The total distance moved per 10 minute time-bin was automatically measured  with EthoVision XT  and used to determine the activity level of the larvae. Knockdown of both dec1 and per2 genes resulted in a noticeably disrupted circadian locomotor activity pattern. 

Zebrafish research with DanioVision

Larvae activity and movement patterns are basic measurements used in many studies. They can reveal information on stereotypic and epileptic behaviors, circadian rhythmicity, motor control, movement disorders, neural development, and more.

DanioVision is a complete system designed for exactly these types of experiments with zebrafish larvae, and is often used in studies related to drug development, safety pharmacology, behavioral genetics, and circadian rhythmicity.

Free demo

Further reading

Ben-Moshe, Z.; Alon, S.; Mracek, P.; Faigenbloom, L.; Tovin, A.; Vatine, G.D.; Eisenberg, E.; Foulkes, N.S.; Gothilf, Y. (2014). The light-induced transcriptome of the zebrafish pineal gland reveals complex regulation of the circadian clockwork by light. Nucleic Acids Research42, 3750-3767.

Read about more studies on circadian rhythmicity in zebrafish in this other case about casein kinase and rhythmicity.