EntoLab

Resources

EntoLab technology

  • Jongsma, M.A.; Thoen, M.P.M.; Poleij, L.M.; Wiegers, G.L.; Goedhart, P.W.; Dicke, M.; Noldus, L.P.J.J.; Kruisselbrink, J.W. (2018). An integrated system for the automated recording and analysis of insect behavior in T-maze arrays. Frontiers in Plant Sciences, 10:20. http://dx.doi.org/10.3389/fpls.2019.00020
  • Kloth, K.J.; Ten Broeke, C.J.M.; Thoen, M.P.M.; Den Brink, M.H.V.; Wiegers, G.L.; Krips, O.E.; Noldus, L.P.J.J.; Dicke, M.; Jongsma, M.A. (2015). High-throughput phenotyping of plant resistance to aphids by automated video tracking. Plant Methods, 11:4. http://dx.doi.org/10.1186/s13007-015-0044-z
  • Thoen, M.P.M.; Kloth, K.J.; Wiegers, G.L.; Krips, O.E.; Noldus, L.P.J.J.; Dicke, M.; Jongsma, M.A. (2016). Automated video tracking of thrips behavior to assess host-plant resistance in multiple parallel two-choice setups. Plant Methods, 12:1. http://dx.doi.org/10.1186/s13007-016-0102-1


Screening plants for insect resistance

  • Kloth, K.J. (2016). Mapping moves on Arabidopsis: From natural variation to single genes affecting aphid behaviour. PhD thesis, Wageningen University, 272 pp. https://edepot.wur.nl/370445 
  • Kloth, K.J.; Busscher-Lange, J.; Wiegers, G.L.; Kruijer, W.; Buijs, G.; Meyer, R.C.; Bouwmeester, H.J.; Dicke, M.; Jongsma, M.A. (2017). Sieve Element-lining Chaperone 1 restricts aphid feeding on Arabidopsis during heat stress. The Plant Cell, 29, 2450-2464. http://dx.doi.org/10.1105/tpc.16.00424
  • Kloth, K.J.; Wiegers, G.L.; Busscher-Lange, J.; van Haarst, J.C.; Kruijer, W.; Bouwmeester, H.J.; Dicke, M.; Jongsma, M.A. (2016). WRKY22 promotes susceptibility to aphids and modulates salicylic acid and jasmonic acid signalling. Journal of Experimental Botany, 67, 2283-3396. http://dx.doi.org/10.1093/jxb/erw159
  • Thoen, M.P.M. (2016). Host-plant resistance to western flower thrips in Arabidopsis. PhD thesis, Wageningen University, 195 pp. https://edepot.wur.nl/387275


Screening beneficial insects

  • De Bruijn, J.A.C. (2020). Information reliability shaping parasitoid foraging behaviour. PhD thesis, Wageningen University, 181 pp. https://edepot.wur.nl/506702
  • De Bruijn, J.A.C.; Vet, L.E.M.; Jongsma, M.A.; Smid, H.M. (2018). Automated high-throughput individual tracking system for insect behavior: Applications on memory retention in parasitic wasps. Journal of Neuroscience Methods, 309, 208–217. http://dx.doi.org/10.1016/j.jneumeth.2018.09.012


Recent blog posts

Screening insecticides for efficacy or tolerance

Screening insecticides for efficacy or tolerance

Maarten Jongsma and colleagues use EntoLab to detect changes in insect behavior that provide a sensitive method for identifying and quantifying resistance development. They tested this approach with pyrethrins against aphids.
Screening plants for resistance to insect pests

Screening plants for resistance to insect pests

EntoLab is a computerized system for automated screening of plants for resistance to sucking insects. It can accurately screen many different plant lines in a high-throughput manner.
Identification of an aphid resistance gene in Arabidopsis

Identification of an aphid resistance gene in Arabidopsis

Karen Kloth used EntoLab in her research on the genetic basis of plant resistance to aphids. Genome-wide association mapping of aphid behavior on 350 natural Arabidopsis accessions led to the discovery of a new gene.