EntoLab

Resources

EntoLab technology

  • Jongsma, M.A.; Thoen, M.P.M.; Poleij, L.M.; Wiegers, G.L.; Goedhart, P.W.; Dicke, M.; Noldus, L.P.J.J.; Kruisselbrink, J.W. (2018). An integrated system for the automated recording and analysis of insect behavior in T-maze arrays. Frontiers in Plant Sciences, 10:20. http://dx.doi.org/10.3389/fpls.2019.00020
  • Kloth, K.J.; Ten Broeke, C.J.M.; Thoen, M.P.M.; Den Brink, M.H.V.; Wiegers, G.L.; Krips, O.E.; Noldus, L.P.J.J.; Dicke, M.; Jongsma, M.A. (2015). High-throughput phenotyping of plant resistance to aphids by automated video tracking. Plant Methods, 11:4. http://dx.doi.org/10.1186/s13007-015-0044-z
  • Thoen, M.P.M.; Kloth, K.J.; Wiegers, G.L.; Krips, O.E.; Noldus, L.P.J.J.; Dicke, M.; Jongsma, M.A. (2016). Automated video tracking of thrips behavior to assess host-plant resistance in multiple parallel two-choice setups. Plant Methods, 12:1. http://dx.doi.org/10.1186/s13007-016-0102-1


Screening plants for insect resistance

  • Kloth, K.J. (2016). Mapping moves on Arabidopsis: From natural variation to single genes affecting aphid behaviour. PhD thesis, Wageningen University, 272 pp. http://edepot.wur.nl/370445 
  • Kloth, K.J.; Busscher-Lange, J.; Wiegers, G.L.; Kruijer, W.; Buijs, G.; Meyer, R.C.; Bouwmeester, H.J.; Dicke, M.; Jongsma, M.A. (2017). Sieve Element-lining Chaperone 1 restricts aphid feeding on Arabidopsis during heat stress. The Plant Cell, 29, 2450-2464. http://dx.doi.org/10.1105/tpc.16.00424
  • Kloth, K.J.; Wiegers, G.L.; Busscher-Lange, J.; van Haarst, J.C.; Kruijer, W.; Bouwmeester, H.J.; Dicke, M.; Jongsma, M.A. (2016). WRKY22 promotes susceptibility to aphids and modulates salicylic acid and jasmonic acid signalling. Journal of Experimental Botany, 67, 2283-3396. http://dx.doi.org/10.1093/jxb/erw159
  • Thoen, M.P.M. (2016). Host-plant resistance to western flower thrips in Arabidopsis. PhD thesis, Wageningen University, 195 pp. https://edepot.wur.nl/387275


Screening beneficial insects

  • De Bruijn, J.A.C.; Vet, L.E.M.; Jongsma, M.A.; Smid, H.M. (2018). Automated high-throughput individual tracking system for insect behavior: Applications on memory retention in parasitic wasps. Journal of Neuroscience Methods, 309, 208–217. http://dx.doi.org/10.1016/j.jneumeth.2018.09.012


Recent blog posts

screening-insecticides

Screening insecticides for efficacy or tolerance

Maarten Jongsma and colleagues use EntoLab to detect changes in insect behavior that provide a sensitive method for identifying and quantifying resistance development. They tested this approach with pyrethrins against aphids.
screening-insect-resistance-aphids

Identification of an aphid resistance gene in Arabidopsis

Karen Kloth used EntoLab in her research on the genetic basis of plant resistance to aphids. Genome-wide association mapping of aphid behavior on 350 natural Arabidopsis accessions led to the discovery of a new gene.
insect-resistance

Screening plants for resistance to insect pests

EntoLab is a computerized system for automated screening of plants for resistance to sucking insects. It can accurately screen many different plant lines in a high-throughput manner.