• Parkinson’s neurorestorative drugs

There is still no cure for Parkinson’s. Recent studies have been focusing on neurorestoration. A recent study from Cronin and Grealy (Neuroscience, 2017) investigates several existing drugs, like Isradipine (used for high blood pressure), Minocycline (antibiotic), and Rasagiline (normally used in conjuction with PD drug Levodopa).

Locomotor functioning of PD zebrafish model

In order to test whether isradipine, minocycline, and rasagiline could protect or restore locomotor functioning after treatment with 6-OHDA (a well-known model for Parkinson’s), the zebrafish larvae were tested using DanioVision.

Individual larvae were placed in a 96-well plate, and their activity was measured during a 50-minute period at 5 days post fertilization, with 10-minute alternating light-dark cycles. The researchers used EthoVision XT to calculate mean distance moved per minute and total distance moved.

Locomotor function restoration

Researchers found that rasagiline restored locomotor function in 6-OHDA-treated zebrafish. It also restored dopaminergic neurons. Minocycline also restored locomotor function, but did not have the restorative effect in the brain. Isradipine showed no significant improvement in either area.

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Zebrafish research with DanioVision

Larvae activity and movement patterns are basic measurements used in many studies. They can reveal information on stereotypic and epileptic behaviors, circadian rhythmicity, motor control, movement disorders, neural development, and more.

DanioVision is a complete system designed for exactly these types of experiments with zebrafish larvae, and is often used in studies related to drug development, safety pharmacology, behavioral genetics, and circadian rhythmicity.

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