12 reasons why you should use DanioVision
Even lighting: precise measurement
Most other systems over- or underestimate animal movement. This is because uneven lighting causes differences in contrast in outer versus inner wells, and shadowing. In turn, this results in bad detection. The DanioVision Observation Chamber has a backlit plate holder that is designed to create even lighting across all wells, preventing these problems.
DanioVision has a unique optical design to prevent angular distortion in the video image of the outer wells. Rather than using fragile mirrors, a robust and compact system provides a straight view into each well. The Fresnel lens does not magnify the image, instead it directs more light straight into the camera without affecting the image quality.
High quality camera
A high quality camera is crucial zebrafish larvae tracking: perfect in resolution, shutter speed, and frame rate for recording the fast “burst-like” movement of zebrafish larvae. Our camera prevents the animal looking like a blurry streak in the video, so there is no misinterpretation of activity.
Smart video storage
Where other systems use little or no compression, DanioVision facilitates long term recordings without running into storage problems. Videos are recorded in MPG format with medium compression for high quality videos without loss of information.
Actually measures animal activity
Instead of detecting general pixel-based changes, DanioVision uses a much more accurate method: in each frame, it detects the whole animal and finds its center point.Variables such as velocity and distance moved are calculated frame to frame.
Infrared based tracking
IR-based lighting and camera allows for tracking in the dark. In contrast to other systems, the wavelength of this IR light is not visible to zebrafish, so it will not bias your measurements.
DanioVision is a complete system including the Observation Chamber and full license of EthoVision XT video tracking software. We also have several add-ons, such as stimuli or temperature control.
Temperature control and preventing overheating
Include the Temperature Control Unit to accurately and safely control the temperature in longer studies. The temperature is measured around the well plate (not in the more distance unit) for high accuracy.
Automation and standardized protocols
Stimuli in the Observation Chamber, such as lights, tapping, or optogenetics, are configured, controlled, and logged by EthoVision XT, which also allows for easy automated data analysis. No need for extra software or manual interference.
Catered to zebrafish larvae studies
EthoVision XT video tracking provides easy experiment setup, optimal detection settings, and elaborate analysis options, specifically for zebrafish larvae studies. You can use or tweak our predefined templates or reuse your settings from previous experiments.
Video track everything
While originally designed for zebrafish larvae, DanioVision is suitable for all small organisms such as insects and (aquatic) invertebrates, in any type of multi-well plate, petri dish, or other small container.
The video tracking software EthoVision XT is also suitable for any other animal (adult fish, rodents, even farm animals). It also includes free analysis on an unlimited number of computers. You get a lot of extra use out of your license!
Highly validated tools
The numbers say it all: we have over 30 years of experience in video tracking zebrafish and zebrafish larvae. EthoVision XT video tracking is used in more than 2,500 labs worldwide and validated in over 34,000 publications. We have installed nearly 300 DanioVision systems, and it has been validated in 400 publications.