12 reasons why you should use DanioVision
Optimal lighting conditions
The DanioVision Observation Chamber has a backlit plate holder that is designed to create even lighting conditions across all wells. This is very important, as differences in contrast in outer versus inner wells, as well as shadows caused by uneven lighting, can both introduce bias in the actual activity measurement. In contrast to other commercially available systems, there is no over or underestimation of animal movement due to contrast differences or shadowing in DanioVision.
Distortion free image of each well
DanioVision also has a unique optical design to prevent angular distortion in the video image of the outer wells. Rather than using fragile mirrors that may deform over time, DanioVision has a Fresnell lens that provides a straight view into each well. This design also makes for a robust and compact system.
High quality camera suited for fast movement
Another key ingredient for reliable video tracking is a high quality camera that is suitable for the fast “burst-like” movement of zebrafish larvae. To prevent recording the animal as a blurry streak in the video (causing a misinterpretation of activity), the DanioVision camera is specifically suited to this type of movement – in resolution, shutter speed, and frame rate.
Actual animal activity measurements
EthoVision XT video tracking is based on the detection and tracking of the actual animal, not the general (pixel-based) activity taking place in the well plate. For these types of experiments, this is a much more accurate method than most other systems use.
Infrared based tracking
Infrared (IR) lighting combined with the IR-sen-sitive camera allows for tracking in the dark. In contrast to other systems, the wavelength of this IR light is not visible to zebrafish, so it will not bias your measurements. Using IR light also makes sure that the white light you might use for a day/night rhythm or as stimulus, does not influence your tracking.
DanioVision is a complete plug-and-play solution, based on an Observation Chamber and full license of EthoVision XT video tracking software. We have several add-on options to make sure you get an all-in-one solution that is perfectly suited for your experiments. The DanioVision Observation Chamber also allows for customization due to its spacious design.
Temperature control and protection against overheating
The Observation Chamber provides a safe and controlled environment designed to prevent overheating. The Temperature Control Unit (optional) allows to you to control the temperature in longer studies.
Stimuli in the Observation Chamber, such as lights, tapping stimuli, or optogenetics, are programmed and controlled by EthoVision XT, which also allows for easy data integration. No need for extra software or manual interference.
Software for all your experiments
The included EthoVision XT full license can be used for other experiments with adult fish (such as in a T-maze or novel tank diving), or even other species such as rodents. As a result, you can get a lot of extra use out of your purchase. It’s also good to know that an EthoVision XT license includes free analysis on an unlimited number of computers.
Catered to zebrafish larvae studies
EthoVision XT has a specially designed DanioVision mode, including easy experiment setup, optimal detection settings, and elaborate analysis options. You can use or tweak our predefined templates or reuse your settings from previous experiments.
Highly validated tools
We have over 30 years of experience in video tracking zebrafish and zebrafish larvae. Our EthoVision XT software is the best video tracking tool on the market and the most validated with over 10,000 publications.
Use across species
While originally designed for zebrafish larvae, DanioVision is suitable for all small organisms in any type of multi-well plate, petri dish, or other small container. Again, the design assures even lighting for accurate video tracking across the testing arena(s).