"For our research, EthoVision XT is an essential tool for sophisticated analysis of behavior as it relates to neural circuit function"
Garret Stuber, University of North Carolina at Chapel Hill, UNC Neuroscience center, Chapel Hill, USA
“EthoVision software has dramatically improved all areas of behavior testing”
Joshua Matulonis, Research Foundation at Stony Brook University, USA
“Tracking software is in the forefront of scientific studies, but various software platforms are either not reliable or very difficult to use. EthoVision XT provides extremely reliable software with an interface that allows everyone to easily track animals” - Erik R. Duboue, New York University, USA
“The Heartbeat detector is magic !”
Prescilla Perrichon, IFREMER – La Rochelle – France
"A great tool for tracking very small insect larvae under IR conditions"
Nick Birch, Scottish Crop Researcher Institute, Dundee, UK
"We successfully use EthoVision and plan to use The Observer in practice for our students"
Marina Pleskacheva, Moscow State University, Lab. Of Physiology, Genetics and Behavior, Moscow, Russian Federation
"EthoVision saves time, perhaps lives. In effect, PhD students can do more research in less labor-intensive time"
Samuel Adeosun, University of Mississippi Medical Center, Jackson, USA
"EthoVision XT is a great product, EthoVision just gets better and better"
Thomas van Groen, University of Alabama at Birmingham, Cell Biology department, Brimingham, USA
“EthoVision is user-friendly and incredibly versatile: it can be used for a wide variety of behavioral experiments, across different lighting conditions (and coat colors), and can track from a range of distances between camera and subject.” - Jerome Pagani, Behavioral Neuroscientist, USA
"EthoVision allows me to track videos accurately and reliably. Now I can use the hours that I used to spend with timers, pen and paper working on other areas of my research"
Jacqueline Womersley, University of CapeTown, Department of Human Biology, Cape Town, South Africa
“This is the best tracking system for open field behavior!”
David Eilam, Tel-Aviv University, Zoology Department, Israel
“A truly useful software to understand animal behavior”
Allan V. Kalueff, Department of Pharmacology, Tulane University Medical School, USA
“EthoVision XT has a convenient tracking/video system with excellent data analysis options and it is fairly intuitive to use”
Diane Lim, University of Pennsylvania, USA
“Really nice tracking program”
Matt Andrzejewski, University of Wisconsin-Madison, USA
“EthoVision XT has allowed our lab to collect rich behavioral data that would have been impossible with our analog system”
Joshua Herrington, Florida International University, USA
“This set of programs allows for flexibility of behavioral observations, scoring and data analyses”
Craig Kinsley, University of Richmond, USA
“EthoVision XT is a powerful tool to analyze insect and spider behavior including tiny little dwarf spiders. Despite of the numerous settings, the intuitive program design allows students to quickly get into the program.”
Roman Bucher, University of Koblenz-Landau, Germany
“DanioVision is an excellent system! We have been using it for two years now, to study both medaka and trout early life stages, and got great results.”
Prof. Cachot, University of Bordeaux, France

Data analysis

EthoVision data analysis Powerful tools

EthoVision XT is powerful in data collection, but of course you will need to analyze this data. EthoVision XT helps you out in that department, too. It has powerful tools for data selection, visualization of your data, and analysis of your data.    

 

 

 

In-depth analysis of your data

Visualizing your data – in data tables, plotted in graphs, and superimposed on your video file – is an important step in analysis. In fact, you can perform this before or after data selection, or even in the data acquiring process.

See what data visualization looks like, and what the possibilities are with EthoVision XT in this video.

If you are interesting in certain behaviors such as body elongation or mobility, you can use the visualization function to fine-tune the thresholds for these behaviors.
Right after data acquisition, use visualization to get a good feel for your data. After data selection and analysis, visualization is a great way to illustrate certain results and present your data.

 

heatmapBuilt-in heatmaps

In addition to integrated visualization, a heatmap gives you an intuitive and unique view of your data. With one glance, you are able answer the question: in which part of the arena did the animal spend most of its time? You can select the data you want to appear in your heat map the same way you always select your data (see below). Use time intervals or nest different periods within a trial. Then sort a series of heatmaps in such a way that you can easily compare between trials, between individuals, or between groups.

You can also adjust your heatmap to your likings. Show the video image on the background, or not. Adjust the size of the blob and precision according to the size of your animal, or change the transparancy or the colors. Of course, you can easily export your heatmaps for use in presentations of scientific papers in all standard formats (PNG, JPG, BMP, GIF).

 

EthoVision data analysis Data selection

You can zoom in into tracks or parts of tracks by the powerful data selection criteria EthoVision XT offers. Selecting data in which animals received a specific treatment allows you to only analyze those data and ignore others. Or nest your data on time, zone or a behavioral state. The results can be visualized in a time-event plot together with the track data and the video file. This way you get an instant feel for your data. Analysis results are also represented in a table. Of course you can export your (raw) track data for further analysis with statistical software programs.

 

 

Data analysis

The number of parameters that can be used is substantial, including trial duration, in zone, distance to zone, distance to zone border, distance moved, velocity, heading, turn angle, angular velocity, mobility, proximity, rotations, elongation, etc. Statistics include mean, variance, standard deviation, standard error, minimum, maximum, sum, total, number of samples, etc.

EthoVision XT data selection and analysis

For instance: use elongation, mobility, and head direction parameters when you are performing a novel object recognition test, a plus maze test, or a fear conditioning test. The stretch-attend posture, characterized by elongation of the animal’s body, is often considered a response to an object that incites fear or curiosity. Additionally, time spent immobile serves as a measure of fear. The rotation parameter is very useful in research on brain defects. 

Of course you can export your (raw) track data for further analysis with statistical software programs.

 

Batch analysis and automatic analysis

In addition to batch acquisition, EthoVision XT also offers batch analysis and automatic analysis, which will save you a lot of time. 

If you have large data files, it may take a while to process them. You can select which trial you want to subject to which data and analysis profiles beforehand and then EthoVision XT runs all the analyses sequentially. You can combine this with batch acquisition of your video files. Let EthoVision XT do the work while you concern yourself with other things - you don’t need to come back and start each separate track or analysis. 

 

group statistics and chartsGroup statistics and charts

You can also compare the results between groups. For example, your experiment exists of two phases: training and testing. You are testing two different doses of a drug and of course, a control group. In total, that means you have six groups you might want to compare. EthoVision XT automatically groups the results. Data are displayed in tables and graphs, giving you an immediate feel for your results and making it easy to spot trends. Within these data, you can also fragment more using time bins or looking at the results per zone.

You can organize these graphs as you wish and adjust the design: you might want to compare treatment groups alongside or maybe you are interested in differences within treatment groups between training and testing. You can export your graphs in all standard formats (PNG, JPG, BMP, GIF).

 

Analysis of hardware events

If you are using the Trial & Hardware Control Module to control external equipment, EthoVision XT enables you to analyze these hardware events. This data can include signals that were sent out from EthoVision XT to external devices (such as to drop a pellet on turn on the house light) and signals that are sent back to EthoVision XT (such as the confirmation of a pellet drop or feedback from the running wheel). This helps you to answer questions such as: “How long was the house light on?” and “How much running did the mouse do?” in combination with the tracking data.

  

Data smoothing

In any tracking system, there are three sources of noise that potentially affect the values of dependent variables such as distance moved or velocity; system noise, outliers, and small movements of the animal (body wobble). That is why you might want to smooth out your data. EthoVision XT has two methods for that. First is the Lowess filter, which fits a curve to the data set using a modified least square regression. The second method uses the defined ‘minimal distance moved’ to smooth out jitter and body wobble.