Zebrafish embryos and larvae are a common model for toxicology research today. From screening compounds to the investigation of developmental toxicity mechanisms, these assays provide an investigative tool that can test a much larger number of compounds compared to mammalian studies.
Toxicity of quantum dots
To effectively test the effects on locomotor activity in response to certain compounds, zebrafish larvae can be loaded into multi-well plates. For example, Duan et al. (2013) investigated the developmental toxicity of quantum dots in larvae in 24-well plates using a DanioVision system.
Duan looked at activity levels of 6 dpf larvae swimming in visible light and found that the total swimming distance was suppressed in treated larvae in a dose-dependent manner. In a light-dark experiment (10-minute bouts of alternating light conditions), the control group showed the typical increased activity levels during the dark phases, while the treated larvae showed significant hypo-activity. Read more about this research on our blog.
DanioVision facilitates the high-throughput screening of zebrafish larvae. In addition to the DanioVision system, Noldus offers another software called DanioScope which automatically measures embryo activity and embryo or larvae heartbeat, flow, and morphology.
Want to see more studies that used DanioVision? Click here for a list of recent publications.